|
| |
首頁 > 抗體産品 > Anti V5 Monoclonal Antibody
Anti V5 Monoclonal Antibody
| 說明書下載 |
Calatog Number: | LT9992 |
Lot Number: | 411005100208 |
Isotype: | Mouse IgG1 |
Clonality: | E10 |
Quantity: | 50 μg |
Immunogen: | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH |
Specificity: | Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins |
Purification: | Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor |
Applications: | Dot, ELISA, IP, IS, WB |
Sensitivity: | WB (with ECL): 1-2ng of V5-tagged fusion proteins |
Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
Images: | Fig. 1:000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2) |