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首頁 > 抗體産品 > Anti GFP Monoclonal Antibody

Anti GFP Monoclonal Antibody

¥1350
Calatog Number:LT9994
Lot Number:411005100210
Isotype:Mouse IgG1
Clonality:GF28R
Quantity:50 μg
Immunogen:GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Specificity:Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Purification:Protein A affinity chromatography from mouse ascites fluid
Applications:Dot, ELISA, IP, IS, WB
Sensitivity:WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
Working Condition:WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-GFP mAb dilution probed against HEK293 cells transfected with GFP-tagged protein vector
Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1µg (2) and 10µg (3) of cell lysates used