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首頁 > 抗體産品 > Anti GAPDH Monoclonal Antibody
Anti GAPDH Monoclonal Antibody
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| Calatog Number: | LT9995 |
| Lot Number: | 411005100211 |
| Isotype: | Mouse IgG1 |
| Clonality: | GA1R |
| Quantity: | 50 μg |
| Immunogen: | Recombinant GAPDH |
| Specificity: | Recognizes native and denatured forms of GAPDH (~37kDa) |
| Cross Reactivity: | GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable |
| Purification: | Protein A affinity chromatography from mouse ascites fluid |
| Applications: | Dot, ELISA, IS, WB |
| Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
| Images: | 
Fig. LEFT: 1:2000 (0.5µg/mL) Ab dilution used in WB of 5µg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3) |
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