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首頁 > 抗體産品 > Anti ERK1 (E19) Monoclonal Antibody
Anti ERK1 (E19) Monoclonal Antibody
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Calatog Number: | LT9998 |
Lot Number: | 411005100214 |
Isotype: | Mouse IgG1 |
Clonality: | E19 |
Quantity: | 50 μg |
Immunogen: | Recombinant full length ERK1 protein |
Specificity: | Recognizes only the ERK1 protein (e.g.: from human and mouse cells) |
Cross Reactivity: | WB: ERK1 from human and mouse cells. IP: ERK1 from human cells. ERK1 from other species may also be detectable |
Purification: | Protein A affinity chromatography from mouse ascites fluid |
Applications: | IP, WB |
Working Condition: | WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test |
Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
Images: | Fig. LEFT: 1:10,000 (0.1µg/mL) Ab dilution used in WB of HEK293 cell lysate; 1µg (1), 5µg (2), and 10µg (3) of cell lysate used; RIGHT: IP of anti-ERK1 using 50µg of Jurkat cell lysate; 0.5µg (1), 1µg (2), and 2µg (3) of anti-ERK1 used |