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首頁 > 抗體産品 > Anti β-Actin Monoclonal Antibody
Anti β-Tubulin Monoclonal Antibody
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| Calatog Number: | LT9991 |
| Lot Number: | 411005100208 |
| Isotype: | Mouse IgG2a |
| Clonality: | BT7R |
| Quantity: | 50 μg |
| Immunogen: | β-Tubulin N-terminal peptide-KLH conjugates |
| Specificity: | Recognizes native and denatured forms of β-Tubulin (~50kDa) |
| Cross Reactivity: | β-Tubulin from human, monkey, mouse, rat, rabbit, chicken. β-Tubulin from other species may also be detectable |
| Purification: | Protein A affinity chromatography from mouse ascites fluid |
| Applications: | Dot, ELISA, IS, WB |
| Working Condition: | WB (with ECL): 1:2000-5000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test |
| Storage: | Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C (add 0.05% NaN3) for several days to weeks |
| Images: | 
Fig.01 LifeTein’s three loading control mAbs reacting against 10μg/lane of mouse brain tissue lysates. 50kD band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5μg/mL); 42kD band is Anti-β-Actin (BA3R) at 1:1000 dilution (1μg/mL); 37kD band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2μg/mL)
Fig.021:4000 (0.25μg/mL) Ab dilution used in WB of 10μg/lane mouse brain tissue lysates |
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